Nsepy is a library to extract historical and realtime data from nses website. In fact, many of the functions in r are actually functions of functions. Novel interventional management of hepatic hydatid cyst. Pulse power technology using nanosecond pulsed electric fields nspefs offers a new stimulus to modulate cell functions or induce cell death for cancer cell ablation. This article on a gene on human chromosome 20 is a stub. Upregulation of pdgf mediates robust liver regeneration. Cancer treatment has been at the forefront of investigations thus far as nspefs permeabilize cellular and intracellular membranes leading to apoptosis and necrosis. The package provides a set of wrappers around several r packages, which give access to more than thirty nse estimators, including batch means estimators geyer, 1992, section 3. Pdf pulse power technology using high intensity up to 300 kvcm nanosecond pulsed electric. Selective susceptibility to nanosecond pulsed electric. A causative nonsense mutation was validated by western blot analysis, strongly suggesting that the bgh phenotype results from the loss of spef2 function. Mechanisms of nanosecond pulsed electric field nspefinduced. Making the most of the spef r trends in sound assessment practice. Spef has then been derived with this model for a spv source connected to a synchronous generator sg by a cable with the nonlinear load.
Effects of nanosecond pulsed electric fields nspef on human prostate cancer cell line lncap. In this study, we sought to determine more specifically the effects of nspef on cell structure and function by exposing human cells to nspef and analyzing them at the cellular and molecular levels. Spef2 is expressed in all ciliated cells and is required for cilia function. Nspef can generate pulsed high voltage electric field in ultra short nanosecond. Pdf nanosecond, highintensity pulsed electric fields. The revoscaler library is a collection of portable, scalable, and distributable r functions for importing, transforming, and analyzing data at scale. Experimental nanopulse ablation of multiple membrane. Jurkat cells showed cytoskeleton, nuclear membrane and telomere damage that coincided with reduced cell survival compared to two adherent cell lines. The power query m function reference includes articles for each of the over 700 functions. Nanosecond pulsed electric fields nspefs regulate phenotypes. Cells can be driven into cell death subroutines that include necrotic features 28, 29.
To learn more about functions and how they work in an expression, see understanding power query m functions. The recipe is implemented as a single r expression that uses the values of the arguments to compute the result. A specialized setup for nspef exposure of individual cells in. Fits semiparametric regression models for panel count survival data. The laboratory follow up of infection and liver function post nspef treatment. Dielectrophoresisdep of spherical cells after nano. R e s u l t s a n d d is c u s s io n pre vious publications describe important aspects of the beha vior of the ep local models, assigned to the tw o systems 733. Nanosecond pulsed electric field nspef ablation as an. In order to do this, state space model of the spv system with vsg controller has been developed. Nanosecond pulsed electric fields nspefs have a variety of applications in the biomedical and biotechnology industries. Nspef functioned safely and effectively as an antitumor therapy in vivo. In this study, we found that after nspef application, the fluorescence lifetime became. The effects of pulsed electric fields have been a period of study since past few decades, which was mainly on the electroporation, bacterial decontamination and biofouling. The laboratory follow up of infection and liver function post nspef treatment because the hydatid cysts in our study is the ce1 type cysts, the complete cyst wall protects the inner parasite antigens from the hosts immune system, serological test on immunological antibodies against specific parasite antigens such as protoscolex and cyst fluid were negative.
Navigating the spefr the language of the spefr other components of the spefr package. Besides, the impact of nspef ablation on liver function should also be taken into consideration in the process. The ts function will convert a numeric vector into an r time series object. Tmem16f has been reported to function as a swellingactivated cl 38, outwardly rectifying cl 39, and ca2activated cl channel 4043. Making the most of the spefr trends in sound assessment practice. Mice bearing 4050 mm 3 b16f10 t umors were treated with either 500 or 750, 200ns pulses 25 kvcm. Wangyans augmented estimating equations aee, aeex, huangwangzhangs method hwz, zhangs maximum. Functions for fitting semiparametric regression models for panel count survival data. Pdf nanosecond pulsed electric field nspef effects on cells and. Effects of nanosecond pulsed electric fields on the intracellular. Impact of vsg parameters on transient performance of solar. You can use it for descriptive statistics, generalized linear models, kmeans clustering, logistic regression, classification and regression trees, and decision forests.
These results have been obtained under different experimental conditions, which may explain. To study the correlation between nspef induced antitumor immunity and the specific cell death pathways. Multiple pulses enhanced permeabilization, for example, r m in gh3 cells fell. The format is ts vector, start, end, frequency where start and end are the times of the first and last observation and frequency is the number of observations per unit time 1annual, 4quartly, 12monthly, etc. Beebe1,3 1 frank reidy research center for bioelectrics, old dominion university, norfolk, va, usa 2 department of.
However, little is known about their effects on the differentiation of stem cells. Navigating the spef r the language of the spef r other components of the spef r. This site is designed for professional practice educators using the spef r. Becoming familiar with the spef r overview of the spef r package. Nanosecond pulsed electric field nspef longdom publishing sl. Function spef for the system under consideration have also been analyzed for the same purpose. The fault indicator functions a data acquisition unit which allows measured information and. To determine more specifically their effects on cell structure and function, human cells were exposed to high intensity up to 300 kvcm nanosecond 10300 ns pulsed electric fields nspef and. Influence of pulsed electric fields and mitochondria. On basis of the in vitro experiment, nspef could induce cell apoptosis, inhibit cell proliferation, and inactivate metastasis and invasion. Documents from the spefr package developed by the division of occupational therapy, school of health and rehabilitation sciences, the university of queensland should be referenced as follows. Verbridge3, 1department of mechanical engineering, 2department of human nutrition, foods, and exercise, and 3department of biomedical engineering. To further identify the antitumor function of nspef, we performed nspef experiment in the ectopic tumorbearing mice model. Mechanisms and immunogenicity of nspefinduced cell death.
As the pulse durations decrease, effects on the plasma membrane pm decrease and effects on intracellular signal transduction mechanisms increase. Nanosecond pulsed electric fields modulate cell function. Since the antitumor function of nspefs has been validated, we. Cal pains, which are activated by nspefs 20,30, have been. Modulation of apoptosis regulation has recently shown therapeutic promise in the treatment of some diseases, including cancer 31. The spefr at a glance student practice evaluation form. Ablation and protective antitumo r immune response induced by nspef in b16f10 melano ma model. An r function is a packaged recipe that converts one or more inputs called arguments into a single output. However, little is known about how nspef affects liver regeneration while being applied to eliminate liver lesions. Sperm flagellar protein 1 is a protein that in humans is encoded by the spef1 gene.
In this paper, we describe distinct effects of nspef that appear to be largely independent of effects on the outer cell membrane, suggesting the potential to modulate cell function by circumventing the plasma membrane and effecting cell structures and functions from inside the cell. Spefr checklist of things to do before the student arrives. Chen r, chen x, beebe sj 20 nanosecond pulsed electric field. Fitting the second region with a logarithmic function fig. Numerical analysis of split dose protocols for nspefelectroporation. We investigated the effects of nanosecond pulsed electric fields nspef on three human cell lines and demonstrated breakdown of the cytoskeleton, nuclear membrane and chromosomal telomere damage. One of the great strengths of r is the users ability to add functions. Undoubtedly, cells respond to nspefs in diverse and celltypespecific ways. Using a function as one of the arguments in a formula that uses a function is called nesting, and well refer to that function as a nested function. Sperm contain cilia, and a mutation in the spef2 gene can cause male infertility due to immobile sperm. Nanosecond pulsed electric field inhibits proliferation. Spef2 plays an important role in spermatogenesis and flagellar assembly. Cells can be driven into cell death subroutines that include necrotic features 28,29.
Application the fault indicator type spef 3a2 c is used in association with disconnector terminal units dtus for detection of short circuits, earth faults and phase discontinuity in distri bution networks. The impact of ultrashort nanopulse on cellular membrane is of biological significance and thus has been studied intensively. Nanosecond pulsed electric field nspef has emerged as a promising tool for hepatocellular carcinoma ablation recently. These studies describe the effects of nanosecond 10300 ns pulsed electric fields nspef on mammalian cell structure and function. In this paper, we study the impact of nspef ablation on liver function by the measurement of serum levels of ast and alt as well as liver regeneration and relevant molecular mechanisms in vivo. Request permission export citation add to favorites track citation. Although the function of nspefs has been explored in multiple cell lines, including tlymphocytes cell lines, hematologic cell lines and pancreatic. Cardiac myocyte excitation by ultrashort highfield pulses. Nanosecond pulsed electric fields depolarize transmembrane. The 95% confidence interval for ld 50 was determined by calculation of a 95% confidence interval corridor range for the best fit shown by shaded areas in fig. F or the nspef example we used an experimental pulse w aveform 4. Revised for occupational therapy university of queensland. Different from cell study, this ex vivo study aims to investigate the biological effects of nanosecond pulsed electric field nspef on an independent multimembrane parasite, human hydatid cyst, to observe the unique influence of nanopulse on macromembrane structure.
Nanosecond pulsed electric field induced cytoskeleton. Nanosecond pulsed electric fields nspefs can produce more significant biological effects than traditional electric fields and have thus attracted rising attention in developing medical applications based on short pulse duration and high field strength, such as effective cancer therapy. Nanosecond pulsed electric field nspef treatment for. Pdf mechanisms and immunogenicity of nspefinduced cell. Taken together, the data in this study demonstrate that spef2 is required for cilia function and identify a new genetic cause of pcd. Dualfunction of baicalin in nspefstreated hepatocytes and. Journal of orthopaedic surgery and research nanosecond pulsed electric field inhibits proliferation and induces apoptosis in human osteosarcoma xudong miao 0 3 shengyong yin 0 1 zhou shao 1 yi zhang 2 xinhua chen 1 0 equal contributors 1 the department of hepatobiliary and pancreatic surgery, the first affiliated hospital, zhejiang university, collaborative innovation center for. Nspef ablated primary b16f10 melanomas and protected 33% of the animals from tumor challenge. Nanosecond pulsed electric fields enhanced chondrogenic. Excel for microsoft 365 excel for the web excel 2019 excel 2016 excel 20 excel 2010 excel 2007 more. Nspef ablation of cancer targets at least three hallmarks of cancer evasion.
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